RESEARCH ARTICLE
Ameliorative Potential of Natural Antioxidants Against Paraquat-Induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster: A Comparative Study
S. Niveditha, T. Shivanandappa*, S.R Ramesh
Article Information
Identifiers and Pagination:
Year: 2017Volume: 5
First Page: 43
Last Page: 56
Publisher Id: TOBCJ-5-43
DOI: 10.2174/1874847301705010043
Article History:
Received Date: 05/7/2017Revision Received Date: 25/09/2017
Acceptance Date: 26/09/2017
Electronic publication date: 14/11/2017
Collection year: 2017
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background:
Natural antioxidants show neuroprotective potential to protect against neurodegenerative disorders in experimental animals. There is a need to characterize newer promising neuroprotective natural molecules.
Objective:
In the present study, we have compared the neuroprotective activity of 4hydroxyisophthalic acid (DHA-I), a novel natural antioxidant from the roots of Decalepis hamiltonii, with the other natural neuroprotective antioxidants, ellagic acid, quercetin and nicotinamide, against paraquat (PQ) neurotoxicity in D. melanogaster.
Results:
Flies exposed to multiple (sub-lethal) dose of PQ showed movement disorder characteristic of Parkinson’s disease (PD). The four natural antioxidants showed ameliorative effects against PQ neurotoxicity in the sub-acute model as seen in survivability, locomotor activity as well as oxidative stress markers including reactive oxygen species (ROS), lipid peroxidation and the endogenous antioxidant defenses.
Conclusion:
Our study shows that the antioxidant compounds exhibit varying degrees of protection against PQ-induced oxidative stress and neurotoxicity with DHA-I, quercetin, and nicotinamide being the most effective and ellagic acid, the least potent in Drosophila. Our results show that mitochondrial Mn-SOD is a critical target for PQ neurotoxicity and the neuroprotection by the antioxidants involves the attenuation of mitochondrial ROS production and oxidative damage.
